Photophysical Processes of Merocyanine 540 in Solutions and in Organized Media
نویسندگان
چکیده
Photophysical processes of merocyanine 540 (MC540) are studied using picosecond time-resolved emission spectroscopy. The nonradiative rate of MC540 increases from 0.32 × 109 s-1 in dioxane to 8.73 × 109 s-1 in water, i.e., nearly 27 times. This is attributed to the decrease in the barrier of photoisomerization with increase in polarity and the consequent increase in the rate of photoisomerization for MC540. The polaritydependent isomerization is also shown to be the cause for the observed increase in emission intensity and lifetime of MC540 in aerosol-OT microemulsions and aqueous bovine serum albumin solutions.
منابع مشابه
Phospholipid monolayer and liposome application in the determination of the photophysical characteristics of MC 540.
Merocyanine 540 is an anionic fluorescent probe which has a wide range of applications in biomembrane research, having, as it does, a high affinity to the lipid phase of biological membranes. Thus, its interactions with model phospholipid membranes with defined structures are an important starting point in the quantification of its photophysical and photochemical characteristics. In this study,...
متن کاملMerocyanine 540 as a flow cytometric probe of membrane lipid organization in leukocytes.
Merocyanine 540 (MC540) is a fluorescent probe that binds preferentially to membranes with loosely packed lipids. When combined with flow cytometry, it provides a novel methodology for rapidly and quantitatively assessing lipid organization in individual leukocytes. Analysis of cells stained simultaneously with MC540 and 1-[4-trimethylammoniumphenyl]-6-phenyl-1,3,5-hexatriene, to normalize for ...
متن کاملRelation between the organization of spectrin and of membrane lipids in lymphocytes
In lymphocytes, the cytoskeletal protein spectrin exhibits two organizational states. Because the plasma membrane lipids of lymphocytes also display two organizational states, it was asked whether there is a relation between the organization of spectrin and of membrane lipids. When mouse thymocytes were stained with merocyanine 540 (MC540), a fluorescent lipophilic probe that binds preferential...
متن کاملSynthesis, photophysical, photochemical, and redox properties of nitrospiropyrans substituted with Ru or Os tris(bipyridine) complexes.
Photochromic nitrospiropyrans substituted with 2,2'-bipyridine (bpy), [Ru(bpy)3]2+, and [Os(bpy)3]2+ groups were synthesized, and their photophysical, photochemical, and redox properties investigated. Substitution of the spiropyran with the metal complex moiety results in strongly decreased efficiency of the ring-opening process as a result of energy transfer from the excited spiropyran to the ...
متن کاملMerocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum.
The purpose of this study was to evaluate the photosensitizing dye merocyanine 540 (MC540) as a means for extracorporeal purging of Plasmodium falciparum-infected erythrocytes from human blood. Parasitized red blood cells bound more dye than nonparasitized cells, and exposure to MC540 and light under conditions that are relatively well tolerated by normal erythrocytes and normal pluripotent hem...
متن کامل